¹Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency, Indonesia

Email: dyah.subositi@gmail.com

Graptophyllum pictum (L.) Griff. (Daun Ungu) is one of the medicinal plants used in the Scientific Jamu (Jamu Saintifik) formula for hemorrhoids. Standardization of G.pictum is necessary for developing and ensuring the quality of herbal raw materials. The first step in standardizing and improving the quality of these varieties is the collection and characterization of their germplasm. This study aimed to determine the genetic diversity of 45 G. pictum accessions from various locations in Indonesia using the ISSR molecular marker. 26 DNA fragments were detected among accessions using four selected ISSR primers, with 76,9% polymorphism. The similarity index between accessions was calculated using a dice coefficient. The UPGMA algorithm was used to create the dendrogram. Graptophyllum pictum accessions had a similarity index of 83.7-100 percent, indicating low genetic diversity among accessions. This is the first report of G. pictum genetic diversity in Indonesia based on the ISSR marker. Other molecular markers and marker systems could be used to provide comprehensive information in the standardization of G. pictum.