¹Faculty of Agriculture,University of Bengkulu, Indonesia

²Faculty of Agriculture, Universitas Mahasaraswati, Indonesia

Email: mimi_sutrawati@unib.ac.id

Begomovirus is the cause of yellow mosaic disease on Solanaceae, Cucurbitaceae, Leguminoceae, and several types of weeds. Begomovirus on several horticulture crops have been reported in various regions in Indonesia. However, the data about Begomovirus infection in Bengkulu, is still very limited. The aim of this research is to detection of Begomovirus infected pumpkin (Cucurbita moschata) in Bengkulu based on  polimerase chain reaction (PCR) using universal primers for Begomovirus. Based on observations in several cultivated areas of pumpkin in Bengkulu, it was found that systemic yellow mosaic and leaf curl on pumpkin. Leaves samples showing yellow mosaic  and leafcurl symptoms were taken for virus detection using PCR (polymerase chain reaction) with universal primers SPG1 / SPG2 for Begomovirus amplified transcriptional activator protein (TrAp) and replication-associated protein (Rep) sequences in the Begomovirus genome with a size of ± 900 bp. This confirmed that  leaf samples from pumpkin were positively  proved to be infected by Begomovirus. The amplicon was sequenced followed by basic local alignment search tool analysis. It was revealed that the sequence had the highest homology (95.20%) to Squash leaf curl China  Virus sequence from Cucumis sativus (Negeri Sembilan, Malaysia) [EF197940. This is the first report of Squash leaf curl China  Virus infection on pumpkin in Bengkulu.