¹Master Program of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret, Jl. Ir. Sutami 36A Surakarta 57126, Indonesia

²Department of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret, Jl. Ir. Sutami 36A Surakarta 57126, Indonesia

Email: ratriyanto@staff.uns.ac.id

Cecum microbes are important in utilizing feed nutrients and immunity systems in poultry. This study aimed to define the differences in the composition of the genus Collinsella, Coriobacteriaceae UCG-002, and Olsenella in the quail cecum supplemented with betaine. The treatment consisted of 3 levels, namely: control (C); C+0.12% betaine supplementation (B1); and B1–0.12% betaine supplementation (B2). This study used a CRD with three replications. The next-generation sequencing method of the 16S rRNA gene region V3–V4 was applied to view the taxonomy profile of microbes (Threshold: 0.8~1). The relative abundance of the genera Collinsella, Coriobacteriaceae UCG-002, and Olsenella were analyzed using ANOVA and the DMRT test on R software. The results showed that the provision of B1 increased genus Collinsella and Coriobacteriaceae UCG-002 more than C and B2 (p<0.05). A significant decrease occurred in treatment B1 compared to treatment C indicated in the genus Olsenella as a pathogenic bacterium in the quail cecum (p<0.05). The B2 treatment showed the relative abundance of the genera Collinsella, Coriobacteriaceae UCG-002, and Olsenella tended to return to the microbial composition of treatment C. This study concluded that giving B1 improved the genus Collinsella, Coriobacteriaceae UCG-002, and Olsenella in the quail cecum tract.