¹Graduate Study Program of Nutrition and Feed Science, Faculty of Animal Science, IPB University, Bogor 16680, Indonesia.

²Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor 16680, Indonesia Indonesia.

³Animal Feed and Nutrition Modelling (AFENUE) Research Group, Faculty of Animal Science, IPB University, Bogor 16680, Indonesia

⁴National Research and Innovation Agency of Indonesia, Jakarta 10340, Indonesia

Email: mochamaddzakyalifian@gmail.com

This study aims to evaluate the effect of substrate nutrient content level on nutrient expression of Hermetia illucens by using a meta-analysis method. A database was constructed from studies that have reported various substrates with nutrient levels in Hermetia illucens. The nutritional content observed in this study were dry matter (DM), ash, crude protein (CP), true protein (TP) and ether extract (EE). A total of 12 articles were integrated into the database. The compiled database was statistically analyzed using a mixed model methodology. The different studies were considered as random effects, and the nutrient substrate dose was treated as a fixed effect. The statistical model used is the p-value. The significance of an effect is stated when the p-value < 0.05. The results showed that the increase in ash, CP, and TP on the substrate had a significant effect on the increase in nutrient Hermetia illucens (P<0.05) and increasing DM substrate tends to increased DM on Hermetia illucens (P<0.1). The conclusion of this study is that the levels of DM, ash, CP, and TP on the substrate affect the nutritional content of Hermetia illucens larvae.